李翠翠; 郭立忠; 盧偉東; 董偉
青島農業(yè)大學山東省應用真菌省級重點實驗室

【中文摘要】 利用RAPD及SRAP分子標記技術對從國內外收集到的12個真姬菇菌株進行遺傳多樣性分析。結果表明,20條RAPD引物共擴增出多態(tài)性條帶285條,18對SRAP引物共擴增出多態(tài)性條帶176條。在相似系數0.800水平時,RAPD和SRAP分子標記分別將12個真姬菇菌株分為5個和6個類群。兩種方法均適用于真姬菇種內鑒定,而SRAP標記較RAPD標記穩(wěn)定性好,更適于真姬菇的種內鑒定。

【英文摘要】 Randomly amplified polymorphic DNA(RAPD)and sequence-related amplified polymorphisms(SRAP)were used to identify 12 strains of Hypsizygus marmoreus Sing.A total of 285 and 176 polymorphic bands were amplified by 20 RAPD primers and 18 SRAP primer pairs,respectively.Two dendrograms based on UPGMA(unweighted pair group method with arithmetic average)cluster analysis using the amplification data revealed that,at the similarity level of 0.8,the 12 strains clustered into five and six groups,respectively.We conclude that both methods are suitable for discriminating among strains of H.marmoreus,and that the SRAP markers were more effective in reflecting genetic diversity.

【中文關鍵詞】 真姬菇; RAPD; SRAP; 聚類分析
【英文關鍵詞】 Hypsizygus marmoreus; RAPD; SRAP; clustering analysis
【基金】國家科技支撐計劃子課題“秸稈栽培食用菌高產高效技術模式與示范”(編號:2007BAD89B09-15);; 公益性行業(yè)科研專項子課題“非熱滅菌秸稈食用菌培養(yǎng)基關鍵技術研究”(編號:200803033-B0604)的部分研究內容

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年01期 【DOI】CNKI:SUN:SYJB.0.2009-01-005